Differential gene expression profiles of periodontal soft tissue from rat teeth after immediate and delayed replantation: a pilot study
채용권, 신서영, 강상욱, 최성철, 남옥형,
소속 상세정보
채용권 ( Chae Yong-Kwon ) - Kyung Hee University School of Dentistry Department of Pediatric Dentistry
신서영 ( Shin Seo-Young ) - Kyung Hee University Graduate School Department of Dentistry
강상욱 ( Kang Sang-Wook ) - Kyung Hee University School of Dentistry Department of Oral and Maxillofacial Pathology
최성철 ( Choi Sung-Chul ) - Kyung Hee University School of Dentistry Department of Pediatric Dentistry
남옥형 ( Nam Ok-Hyung ) - Kyung Hee University School of Dentistry Department of Pediatric Dentistry
Abstract
Purpose: In dental avulsion, delayed replantation usually has an uncertain prognosis. After tooth replantation, complex inflammatory responses promote a return to periodontal tissue homeostasis. Various types of cytokines are produced in the inflammatory microenvironment, and these cytokines determine the periodontal tissue response. This study aimed to identify the gene expression profiles of replanted teeth and evaluate the functional differences between immediate and delayed replantation.
Methods: Maxillary molars from Sprague-Dawley rats were extracted, exposed to a dry environment, and then replanted. The animals were divided into 2 groups according to the extra-oral time: immediate replantation (dry for 5 minutes) and delayed replantation (dry for 60 minutes). Either 3 or 7 days after replantation, the animals were sacrificed. Periodontal soft tissues were harvested for mRNA sequencing. Hallmark gene set enrichment analysis was performed to predict the function of gene-gene interactions. The normalized enrichment score (NES) was calculated to determine functional differences.
Results: The hallmark gene sets enriched in delayed replantation at 3 days were oxidative phosphorylation (NES=2.82, Q<0.001) and tumor necrosis factor-alpha (TNF-α) signaling via the nuclear factor kappa light chain enhancer of activated B cells (NF-κB) pathway (NES=1.52, Q=0.034). At 7 days after delayed replantation, TNF-α signaling via the NF-κB pathway (NES=?1.82, Q=0.002), angiogenesis (NES=?1.66, Q=0.01), and the transforming growth factor-beta signaling pathway (NES=?1.46, Q=0.051) were negatively highlighted.
Conclusions: Differentially expressed gene profiles were significantly different between immediate and delayed replantation. TNF-α signaling via the NF-κB pathway was marked during the healing process. However, the enrichment score of this pathway changed in a time-dependent manner between immediate and delayed replantation.
키워드
Gene expression profiling; RNA sequencing; Tooth avulsion; Tooth replantation
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